Common problem

John jjmcq at atl.mediaone.net
Sat Mar 10 10:02:42 EST 2001


Way too much DNA.  I've seen it many times before.
cut it 10 fold and run it again.




"Claire Fenech" <c.j.fenech at sheffield.ac.uk> wrote in message
news:94456o$fjt$1 at bignews.shef.ac.uk...
> Hi all
>
> When you do restriction digests then run the digested DNA on an agarose
gel
> - do you ever get "blobby" bands (with lots of "trailing dots") ?
> I sometime get this and it is not prevented by phenol:chloroform
extraction
> after digest, nor by heat denaturing the enzymes.
> Does anybody know what this common phenomenon is ? Is it something to do
> with EtBr interaction ?? Overloading ? Too much DNA ?
> I am curious to know even if it is obvious.
>
> Thanks for your suggestions
>
> --------------------------------------------------------------------------
--
> Claire Fenech, Research Associate
> c.j.fenech at sheffield.ac.uk
> Phone: 0114 222 2364
>
> Institute of Molecular Physiology
> Alfred Denny Building
> University of Sheffield
> Western Bank
> Sheffield S10 2TN







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