random alkaline RNA ladder and RNaseT1 digestion - need help

Dirk M ü ller d.mueller at lb.tu-berlin.de
Mon Mar 12 04:06:02 EST 2001

for analyses of my ribozymes i need to carry out an RNaseT1 digestion and an
alkaline digestion of the RNA to build an RNA ladder. I found protocols in
the literature but as it seems to be normal with published protocols - they
dont't work.
So can anybody help me out with a protocoll that gives good result?

thank you,

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