AFLP: automatic or manual?

Thomas Moen thomas.moen at akvaforsk.nlh.no
Mon Mar 12 04:57:47 EST 2001


We're currently running AFLPs "the old way", using manual sequencing and
radioactive probes. Now, we may get a 377 sequencer at a very reasonable
price. So I was wondering if anyone could help me on the pros and cons of
doing AFLP on automatic sequences. For example, I've heard that it is easier
to distinguish the separate bands if you run manually, especially if the
bands are close to each other. We have to decide on this soon, so I would be
very glad for any advice.

Thanks in advance!

Thomas Moen







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