Digital camera densitometry

Dima Klenchin klenchin at REMOVE_TO_REPLY.facstaff.wisc.edu
Mon Mar 12 13:01:26 EST 2001


Philip Aisen <aisen at aecom.yu.edu> wrote:
:If anyone has had favorable experience with digital camera densitometry 
:of Westerns and SDS gels I would appreciate hearing about the camera and 
:software used.

No fancy setup here. Cheap HP scanner, and any software that
can count pixels in a selected area (NIH image, Adobe Photoshop -
in fact most bitmap editing programs can do it). Whether you have
this simple solution or an expensive specialized setup, in order to 
claim quantitative results you must have internal calibration curve 
on the same gel/blot. As long as calibration is present and is 
reasonable, it completely does not matter how you obtained it - no
matter what, Westerns are intrinsically highly non-linear.

        - Dima






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