gibco restriction enzymes buffer compatibility chart?

Wolfgang Schechinger wolfsc at
Mon Mar 12 20:33:00 EST 2001

in my experience, there were no problems in interchanging buffers 
and enzymes from NEB and Roche. For multiple digets, I normally use 
the buffer system that resembles to me the most convenient. 

As long as you don't use high concentrations of RE (when the 
components of the RE storage buffer may significantly contribute to 
the composition of the digestion mix), you shouldn't experience 


> From:          "Don Walthers" <genejcky at>
> Subject:       gibco restriction enzymes buffer compatibility chart?
> Date:          Mon, 12 Mar 2001 16:08:37 -0800
> Organization:  Oregon Health Sciences University
> To:            methods at

> Hello,
> Is there a datasheet that shows the activity of each Gibco enzyme in the
> different React buffers similar to what NEB has? Or do they not not
> recommened double digests with enzymes that require different buffers? I
> figure I'd ask here before spending even more time on hold with tech
> support. I did not see anything on their web site.
> Thanks,
> --
> Don Walthers, PhD genejcky at
> Dept of Molecular Microbiology
> Oregon Health Sciences University
> Portland OR
> Our gene pool needs a little chlorine.

Dr. Wolfgang Schechinger
Lab N233 (c/o Dr. Steve Roffler)
Institute of Biomedical Sciences
Academia Sinica
Yen-Chio Yuan Rd. Sec.2
Taipei 115
Taiwan R.O.C.
Tel +886-2-2798-9152
Fax +886-2-2782-9142
Mobile +886-925-136893
wolfsc at


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