Biacore X sample injection

Andy Scotter pcxajs at unix.ccc.nottingham.ac.uk
Tue Mar 13 03:22:16 EST 2001


	I usually add 2-3ul of air then 2-3ul sample (additional to the
60ul in your example) then another 2-3ul of air when I inject samples into
a BIAcoreX.  That is enough to get well defined bubbles in the tip either
side of a plug of your sample.  That will prevent any mixing of your
sample with the buffer already in the system so you get better
sensorgrams.

Hope that helps,

Andy

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Andy Scotter
Dept. of Biological Chemistry
School of Chemistry
University of Nottingham
University Park
Nottingham
NG7 2RD
Tel: 0115 9514193
Fax: 0115 9513564
pcxajs at unix.ccc.nottingham.ac.uk

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