Biacore X sample injection

Andy Scotter pcxajs at
Tue Mar 13 03:22:16 EST 2001

	I usually add 2-3ul of air then 2-3ul sample (additional to the
60ul in your example) then another 2-3ul of air when I inject samples into
a BIAcoreX.  That is enough to get well defined bubbles in the tip either
side of a plug of your sample.  That will prevent any mixing of your
sample with the buffer already in the system so you get better

Hope that helps,



Andy Scotter
Dept. of Biological Chemistry
School of Chemistry
University of Nottingham
University Park
Tel: 0115 9514193
Fax: 0115 9513564
pcxajs at


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