how to get rid of rubisco

[Michael Witty]

Dear Ralf,
          if you are doing westerns, what about doing a non-denaturing gel
and seeing if that separates your protein from RUBISCO?  Mike.

On Tue, 13 Mar 2001, Ralf Weigel wrote:

> 
> Dear colleagues,
> 
> the protein of our our interest is only slightly larger than rubisco. In
> 
> our western blots with cytoplasmic protein extracts the signal of  our
> protein of interest is sometimes overlaid by (the highly abundant)
> rubisco, a problem which is not observed with nuclear extracts. Is there
> 
> a quick and simple way to get rid of rubisco in cytoplasmic extracts ?
> Any comments and suggestions are greatly appreciated.
> 
>     Ralf
> --
> 
> Ralf R. Weigel
> 
> Albrecht von Haller Institute of Plant Sciences
> General and Developmental Plant Physiology
> Untere Karspuele 2
> 37073 Goettingen
> Germany
> 
> Tel.:  0551/3919664
> Fax: 0551/397820
> 
> 
> 
>