how to get rid of rubisco
mw132 at mole.bio.cam.ac.uk
Wed Mar 14 03:53:18 EST 2001
if you are doing westerns, what about doing a non-denaturing gel
and seeing if that separates your protein from RUBISCO? Mike.
On Tue, 13 Mar 2001, Ralf Weigel wrote:
> Dear colleagues,
> the protein of our our interest is only slightly larger than rubisco. In
> our western blots with cytoplasmic protein extracts the signal of our
> protein of interest is sometimes overlaid by (the highly abundant)
> rubisco, a problem which is not observed with nuclear extracts. Is there
> a quick and simple way to get rid of rubisco in cytoplasmic extracts ?
> Any comments and suggestions are greatly appreciated.
> Ralf R. Weigel
> Albrecht von Haller Institute of Plant Sciences
> General and Developmental Plant Physiology
> Untere Karspuele 2
> 37073 Goettingen
> Tel.: 0551/3919664
> Fax: 0551/397820
More information about the Methods