Alternate source of DEAE membrane?

Dima Klenchin klenchin at REMOVE_TO_REPLY.facstaff.wisc.edu
Wed Mar 14 09:34:33 EST 2001


hroychow at nmsu.edu ("Dr. Hiranya S. Roychowdhury") wrote:
:
:Hi Dima, have you tried using the cellulosic filter paper. I am afraid it
:will fall apart in the buffer during electrophoresis.

Yes, I did. No, it won't fall apart. The only problem is
small pieces of cellolose, but they can be spun down 
after elution easily.

        - Dima

:At 05:29 PM 3/13/01 GMT, Dima Klenchin wrote:
:>taylor at org.chem.ethz.ch (hilvertg) wrote:
:>:I'm not sure if this has been posted previously, but I am looking for an
:>:alternative source for DEAE membranes for use in DNA
:>:electroelution/purification. I used to use Schleicher and Schuell NA45,
:>:but my supplier says that it is (unfortunately) no longer available from
:>:S&S. Is there another company that makes a similar product?
:>
:>Whatman DE-81. It's a filter paper. Works just fine for electroelution.
:>
:>        - Dima




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