Q: EtBr absorbance

[Jan Aelen]

Krzysztof Wasowicz wrote:

> Hi,
> I have an EtBr solution of unknown concentration (between 10 and 20
> mg/ml). Does anyone know what is the specific absorbance of EtBr
> solution and at what wavelength it should be measured? I cannot make a
> reference solution. Any help will be appreciated. Please, e-mail me.
> Krzysztof Wasowicz, DVM, Ph. D.
> Department of Animal Anatomy
> Faculty of Veterinary Medicine
> University of Warmia and Mazury
> ul. Oczapowskiego 13 (Bldg. 105J)
> 10-957 Olsztyn-Kortowo
> Poland
> Phone: +48-89-5233688, 5233733
> Fax: +48-89-5234986
> Email: wasowicz at uwm.edu.pl

Hi

Look at the catalog of Applichem p.e.

nm max in 50 % MeOH  :  524-527 nm
Ext. 1%/1 cm at max. nm :150-160

--
J. Aelen
Faculty of Science
KUN
The Netherlands
e-mail: Janal at nmr.kun.nl