Subcellular fractions??

Dr. Hiranya S. Roychowdhury hroychow at
Wed Mar 14 21:24:25 EST 2001

Go to the library and locate the "Methods in Enzymology" series. That is
the most authoritative source of the information you are after. Both the
Med School and the main campus libraries have them (depending on where u
r). There may be places on web that have the cell fractionation protocols,
but it may be easier for you to pick out the right application from the
Meth. Enzymol. volumes. They even have cumulative indices. 

At 02:53 PM 3/14/01 -0700, Neal Robert Melvin wrote:
>Can anyone recommend a good website (or text chapter) that discussesthe
>various methods (differential centrifugation, detergent concentrations,
>etc) used to obtain different subcellular components (membrane versus
>cytosol) from a lysate?
>'If sleep does not serve an absolutely vital function, then it is the
>biggest mistake the evolutionary process has ever made'
>- Dr. Allan Rechtschaffen
>Neal Melvin
>University of Calgary - Faculty of Medicine
>Department of Neuroscience - Neuroscience Research Group (NRG)
>Health Sciences Centre
>3330 Hospital Drive, NW
>Calgary, Alberta, Canada
>T2N 4N1
>e-mail: nrmelvin at
>Phone: (403) 220-4490 (lab)
>       (403) 283-8731 (fax)
Dr. Hiranya Sankar Roychowdhury
College Asst. Prof.
Molecular Biology,
Dept. of Chemistry & Biochemistry	
Box 30001 - 3MLS
New Mexico State University
Las Cruces, NM 88003

Lab: (505) 646 4722
Office: (505) 646 8256
hroychow at


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