EtBr absorbance

R. Jayakumar jakku at
Thu Mar 15 04:14:34 EST 2001

   why do you want to know the concentration of ethidium bromide solution.
If it is for staining DNA gels, I don't think you need to bother much about
concentrations whether it be 10 or 20mg/ml.  Just addd a little of this to
water or buffer and you should be able to stain it.  around 10/20ul to 100
ml of solution.

----- Original Message -----
From: "Krzysztof Wasowicz" <wasowicz at>
To: <methods at>
Sent: Wednesday, March 14, 2001 7:27 PM
Subject: Q: EtBr absorbance

> Hi,
> I have an EtBr solution of unknown concentration (between 10 and 20
> mg/ml). Does anyone know what is the specific absorbance of EtBr
> solution and at what wavelength it should be measured? I cannot make a
> reference solution. Any help will be appreciated. Please, e-mail me.
> Krzysztof Wasowicz, DVM, Ph. D.
> Department of Animal Anatomy
> Faculty of Veterinary Medicine
> University of Warmia and Mazury
> ul. Oczapowskiego 13 (Bldg. 105J)
> 10-957 Olsztyn-Kortowo
> Poland
> Phone: +48-89-5233688, 5233733
> Fax: +48-89-5234986
> Email: wasowicz at


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