elimination of DNA from an RNA sample

Dr. Duncan Clark d_clark at nospam.demon.co.uk
Thu Mar 15 05:38:03 EST 2001

In article <98oklv$fm6$1 at fe2.cs.interbusiness.it>, the eminent Stefi~ at
Centro Servizi Interbusiness wrote
>I need to perform an RT-PCR but my DNase I doesn't clean my RNA sample from
>contaminant DNA. I use glass-fiber based kit for extraction and I treat my
>RNA sample with 1U of DNase I every ug of RNA.

Make sure you incubate with an Mn containing buffer and not Mg for the

The problem with being on the cutting edge is that you occasionally get 
sliced from time to time....

Duncan Clark
GeneSys Ltd.
Tel: +44(0)1252376288
FAX: +44(0)8701640382

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