elimination of DNA from an RNA sample

Dr. Duncan Clark d_clark at nospam.demon.co.uk
Thu Mar 15 05:38:03 EST 2001


In article <98oklv$fm6$1 at fe2.cs.interbusiness.it>, the eminent Stefi~ at
Centro Servizi Interbusiness wrote
>I need to perform an RT-PCR but my DNase I doesn't clean my RNA sample from
>contaminant DNA. I use glass-fiber based kit for extraction and I treat my
>RNA sample with 1U of DNase I every ug of RNA.


Make sure you incubate with an Mn containing buffer and not Mg for the
DNase.

Duncan
-- 
The problem with being on the cutting edge is that you occasionally get 
sliced from time to time....

Duncan Clark
GeneSys Ltd.
Tel: +44(0)1252376288
FAX: +44(0)8701640382
http://www.dnamp.com
http://www.genesys.demon.co.uk




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