elimination of DNA from an RNA sample

S.Tiwari bsssmt at bath.ac.uk
Thu Mar 15 08:26:29 EST 2001


Is there any possibility of DNA remaining in the polyA RNA after one round of
oligodt purification?
Response would be a great help!

"Dr. Duncan Clark" wrote:

> In article <98oklv$fm6$1 at fe2.cs.interbusiness.it>, the eminent Stefi~ at
> Centro Servizi Interbusiness wrote
> >I need to perform an RT-PCR but my DNase I doesn't clean my RNA sample from
> >contaminant DNA. I use glass-fiber based kit for extraction and I treat my
> >RNA sample with 1U of DNase I every ug of RNA.
>
> Make sure you incubate with an Mn containing buffer and not Mg for the
> DNase.
>
> Duncan
> --
> The problem with being on the cutting edge is that you occasionally get
> sliced from time to time....
>
> Duncan Clark
> GeneSys Ltd.
> Tel: +44(0)1252376288
> FAX: +44(0)8701640382
> http://www.dnamp.com
> http://www.genesys.demon.co.uk

--
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Sushma Tiwari
Department of Biology and Biochemistry
South Building
University of Bath
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BA2 7AY,
UK.
Phone: 01225 826826 ext 5427
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