EtBr absorbance

Michael L. Sullivan mlsulliv at facstaff.wisc.edu
Thu Mar 15 09:39:32 EST 2001


If you use only 1ul of 10 mg/ml EtBr per 100 ml directly in the gel it
works fine to stain.  AND this generates much less waste.

Mike

>hi...
>   why do you want to know the concentration of ethidium bromide solution.
>If it is for staining DNA gels, I don't think you need to bother much about
>concentrations whether it be 10 or 20mg/ml.  Just addd a little of this to
>water or buffer and you should be able to stain it.  around 10/20ul to 100
>ml of solution.
>    bye
>jayakumar
>

Michael L. Sullivan, Ph.D

U.S. Dairy Forage Research Center
1925 Linden Drive West
Madison WI, 53706

(608) 264-5144 Phone
(608) 264-5147 Fax

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