Alternate source of DEAE membrane?
klenchin at REMOVE_TO_REPLY.facstaff.wisc.edu
Thu Mar 15 09:42:36 EST 2001
hroychow at nmsu.edu ("Dr. Hiranya S. Roychowdhury") wrote:
:Thanx Dima. Indeed we have a bunch of that in the lab and an undergrad
:wondered why we couldn't use it while the NA45 was unavailable.
You have good undergrads...
:it would fall apart while doing the electrophoresis. After the
:electrophoresis it surely wouldn't matter since the cellulose may be simply
:spun down. I am going to ask her to try it now.
:Did u use the DE81 straight, or did u activate it further by a NaOH wash?
Straight. I don't think ionic form of the ion exchanger
matters in this case.
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