Deborah.Hailstones at agric.nsw.gov.au
Thu Mar 15 23:34:32 EST 2001
Do all RNAses degrade all RNAs? Can I buy one which will definitely *not*
degrade double stranded (viral replicative) RNA?
I'm looking to amplify a viral sequence from "dsRNA" extracted from infected
plants, but i suspect they also contain some single stranded plant RNA, and/or
DNA since my "uninoculated" plants are amplifying a band of the same size. The
viral gene I'm trying to amplify is a homolog of a plant gene (family), tho I'm
yet to confirm that the plant gene will amplify under my conditions by doing
just PCR cf RT-PCR.
So, one option may be to remove plant DNA with DNAse, but will RNAse destroy my
dsRNA? If not, how do I then ensure that the RNAse is fully inactivated before
I start my RT?
Any help much appreciated! Cheers
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