Internal His tags

Michael Witty mw132 at
Sat Mar 17 07:18:55 EST 2001

Dear All,
        I want to make a gene and purify a protein by IMAC, but my
protein has N _and_ C terminal processing.  This makes me wonder where
to put the His tag!  Are there examples of proteins that people have
added internal his tags too?  For example on an exposed loop?
Regards, Mike.

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