Internal His tags

[GSzatmari]

Michael Witty wrote in message ...
>Dear All,
>        I want to make a gene and purify a protein by IMAC, but my
>protein has N _and_ C terminal processing.  This makes me wonder where
>to put the His tag!  Are there examples of proteins that people have
>added internal his tags too?  For example on an exposed loop?
>Regards, Mike.
>

Mike,
Yes there is - one I can recall is the work done on FhuA from E.coli.
Check out some of the work from the Coulton group at McGill University.

George