Internal His tags
gszatmari at videotron.ca
Sat Mar 17 11:30:08 EST 2001
Michael Witty wrote in message ...
> I want to make a gene and purify a protein by IMAC, but my
>protein has N _and_ C terminal processing. This makes me wonder where
>to put the His tag! Are there examples of proteins that people have
>added internal his tags too? For example on an exposed loop?
Yes there is - one I can recall is the work done on FhuA from E.coli.
Check out some of the work from the Coulton group at McGill University.
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