Gys de Jongh
GysdeJongh at planet.nl
Sun Mar 18 08:54:52 EST 2001
"Burkhard Hassel" <Burkhard.Hassel at t-online.de> wrote in message
news:3ab20286.1238164 at news.btx.dtag.de...
> On Thu, 8 Mar 2001 12:11:02 +0100, "Arnoud van Vliet"
> <avvliet at knoware.nl> wrote:
> >To get rid of SDS,
> >perform a sodium acetate/ethanol precipitation, or alternatively use a
> >silica-based spin column sold by numerous companies like Qiagen, Promega etc
> No, use a sodium CHLORIDE / ethanol precip. (1/10 vol 2 M NaCl, 2,5
> vol EtOH). With acetate you'll get unsoluble sodium acetate.
if you use sodium acetate the SDS will precipitate.
The SDS stays in solution at high NaCl concentrations.
See maniatis appendix
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