isoamyl alcohol

Gys de Jongh GysdeJongh at planet.nl
Sun Mar 18 08:54:52 EST 2001


"Burkhard Hassel" <Burkhard.Hassel at t-online.de> wrote in message
news:3ab20286.1238164 at news.btx.dtag.de...
> On Thu, 8 Mar 2001 12:11:02 +0100, "Arnoud van Vliet"
> <avvliet at knoware.nl> wrote:
> >To get rid of SDS,
> >perform a sodium acetate/ethanol precipitation, or alternatively use a
> >silica-based spin column sold by numerous companies like Qiagen, Promega etc
> No, use a sodium CHLORIDE / ethanol precip. (1/10 vol 2 M NaCl, 2,5
> vol EtOH). With acetate you'll get unsoluble sodium acetate.


Hi,
if you use sodium acetate the SDS will precipitate.
The SDS stays in solution at high NaCl concentrations.
See maniatis appendix
--
Gys






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