DNA isolation of corn salad

MARCEL QUINT Quint at 350-ipsp.ipsp.Uni-Hohenheim.DE
Tue Mar 20 04:27:07 EST 2001

I am looking for a DNA-isolation protocol for corn salad/lambs lettuce 
(Valerianella locusta). I have tried with the general CTAB-Chloroform 
method, but this results in huge enourmous pellets after isopropanol 
precipitation (just too big). DNA quality is ok, but it is very difficult to 
dissolve in a suitable volume. Maybe some special protocol for any 
vegetable relatives will do better.
Thanks a lot for your help in advance,

Marcel Quint

University of Hohenheim, Germany


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