sequencing from a gel cut-band?

Alan Smith alansmith at students.wisc.edu
Tue Mar 20 15:32:00 EST 2001


One of my lab mates tried this and did not get a sequence read from 36 lanes
on a sequencing gel.  After that we never tried it again.
Alan smith

-----Original Message-----
From: owner-methods at hgmp.mrc.ac.uk
[mailto:owner-methods at hgmp.mrc.ac.uk]On Behalf Of Stefi~
Sent: Tuesday, March 20, 2001 12:57 PM
To: methods at hgmp.mrc.ac.uk
Subject: sequencing from a gel cut-band?


Is it possible to sequence a band from a low melting point agarose gel?
What's the max lenght of a segment to be correctly sequenced? Thx very much.
Stefano
gifalone at tin.it

http://web.tiscalinet.it/gifalone
ICQ 110419911


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