sequencing from a gel cut-band?

Rick A. Bright rbright at
Tue Mar 20 19:32:26 EST 2001

You can isolate the DNA from the gel, then purify with a Qaigen kit and
sequence it very well.  An extra step, but it works well.

""Alan Smith"" <alansmith at> wrote in message
> One of my lab mates tried this and did not get a sequence read from 36
> on a sequencing gel.  After that we never tried it again.
> Alan smith
> -----Original Message-----
> From: owner-methods at
> [mailto:owner-methods at]On Behalf Of Stefi~
> Sent: Tuesday, March 20, 2001 12:57 PM
> To: methods at
> Subject: sequencing from a gel cut-band?
> Is it possible to sequence a band from a low melting point agarose gel?
> What's the max lenght of a segment to be correctly sequenced? Thx very
> Stefano
> gifalone at
> ICQ 110419911
> ---

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