sequencing from a gel cut-band?

Rick A. Bright rbright at emory.edu
Tue Mar 20 19:32:26 EST 2001


You can isolate the DNA from the gel, then purify with a Qaigen kit and
sequence it very well.  An extra step, but it works well.

""Alan Smith"" <alansmith at students.wisc.edu> wrote in message
news:JIEHICBJDMGFLNGJIBDMAEJJCCAA.alansmith at students.wisc.edu...
> One of my lab mates tried this and did not get a sequence read from 36
lanes
> on a sequencing gel.  After that we never tried it again.
> Alan smith
>
> -----Original Message-----
> From: owner-methods at hgmp.mrc.ac.uk
> [mailto:owner-methods at hgmp.mrc.ac.uk]On Behalf Of Stefi~
> Sent: Tuesday, March 20, 2001 12:57 PM
> To: methods at hgmp.mrc.ac.uk
> Subject: sequencing from a gel cut-band?
>
>
> Is it possible to sequence a band from a low melting point agarose gel?
> What's the max lenght of a segment to be correctly sequenced? Thx very
much.
> Stefano
> gifalone at tin.it
>
> http://web.tiscalinet.it/gifalone
> ICQ 110419911
>
>
> ---





More information about the Methods mailing list