cDNA-libraries from small amounts of plant tissue - help, the third

Dr. Klaus Eimert eimert at web.de
Wed Mar 21 02:18:26 EST 2001


Seems I have trouble with my newsreader and/or news server - tried to post 
this message twice, but couldn't see it in the ng. So, here we go again:


Dear Netters,

as the subject states - we are interested in constructing libraries from 
very small amounts of (limited) plant tissue.
 
We would like to ask for your personal experience in this area, like: 
Should we go for total RNA instead of polyA, because of the limited source? 
What are your preferred protocols for isolation (yield vs. quality; kits 
vs. homegrown; etc)? To go for seperate 1st strand synthesis or for all-in-
one-tube? What is your good/bad experience with certain protocols and/or 
kits/companies? 
Any help or pointers to sources are very much appreciated! I could post the 
collected answers (hopefully alot of them) to the group if people are 
interested. 
Thank you very much in advance.

Yours sincerely,

Klaus Eimert


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Dr. Klaus Eimert
Dept. of Botany
State Research Institute Geisenheim             
Von-Lade-Str. 1                                 
D-65366 Geisenheim                             
Germany           
      
Phone:    + 49 6722 502 469
Fax:      + 49 6722 502 460
e-mail:   eimert at web.de
URL: www.mnd.fh-esbaden.de/fag/bio/bo/ebostart.html
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