Immuno and in-situ hybridisation on Plastic resins

M.K.Scott at newcastle.ac.uk M.K.Scott at newcastle.ac.uk
Wed Mar 21 09:38:05 EST 2001


I am looking to try sectioning a plastic resin block and would like to
know if there is a resin available to perform both Immunocytochemistry,
(ICC),  and in-situ hybridisation, (ISH), on these sections.  I have
heard that Methacrylate resin may do ISH and I know ICC works on this
but am not sure of methods.  I am not sure if this is Methyl
Methacrylate or Glycol Methacrylate.
I would appreciate any advice on what resin to use and also on
methodology for both ICC and ISH.

Thanx
Mark K. Scott

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