cDNA-libraries from small amounts of plant tissue - help, the third

S.Tiwari bsssmt at bath.ac.uk
Wed Mar 21 09:35:44 EST 2001


if you get any replies could you forward them to me as well, we both share the
same problem!

"Dr. Klaus Eimert" wrote:

> Seems I have trouble with my newsreader and/or news server - tried to post
> this message twice, but couldn't see it in the ng. So, here we go again:
>
> Dear Netters,
>
> as the subject states - we are interested in constructing libraries from
> very small amounts of (limited) plant tissue.
>
> We would like to ask for your personal experience in this area, like:
> Should we go for total RNA instead of polyA, because of the limited source?
> What are your preferred protocols for isolation (yield vs. quality; kits
> vs. homegrown; etc)? To go for seperate 1st strand synthesis or for all-in-
> one-tube? What is your good/bad experience with certain protocols and/or
> kits/companies?
> Any help or pointers to sources are very much appreciated! I could post the
> collected answers (hopefully alot of them) to the group if people are
> interested.
> Thank you very much in advance.
>
> Yours sincerely,
>
> Klaus Eimert
>
> ---------------------------------------------------------------------------
> Dr. Klaus Eimert
> Dept. of Botany
> State Research Institute Geisenheim
> Von-Lade-Str. 1
> D-65366 Geisenheim
> Germany
>
> Phone:    + 49 6722 502 469
> Fax:      + 49 6722 502 460
> e-mail:   eimert at web.de
> URL: www.mnd.fh-esbaden.de/fag/bio/bo/ebostart.html
> ---------------------------------------------------------------------------

--
*********************************************
Sushma Tiwari
Department of Biology and Biochemistry
South Building
University of Bath
Claverton Down,
BATH,
BA2 7AY,
UK.
Phone: 01225 826826 ext 5427
Fax : 01225 826779
*********************************************

-------------- next part --------------
A non-text attachment was scrubbed...
Name: bsssmt.vcf
Type: text/x-vcard
Size: 180 bytes
Desc: Card for S.Tiwari
Url : http://iubio.bio.indiana.edu/bionet/mm/methods/attachments/20010321/d9c011b8/bsssmt.bin


More information about the Methods mailing list