Total phospholipid assay?
wolfsc at ibms.sinica.edu.tw
Wed Mar 21 11:35:28 EST 2001
first extract all lipids by Bligh and Dyer Method, then perform
an essay for inorganic phosphate (molybdic acid procedure). If I
remember correctly, there should be some papers from Schmitz-G and or
Assmann and or Robenek from 1980 - 1990. Maybe you are able to
locate them in PubMed. Actually, thre should be plenty of references,
in that time and before that was common procedure.
> From: ez <ezclimber at earthlink.net>
> Subject: Total phospholipid assay?
> Date: Wed, 21 Mar 2001 09:05:00 -0700
> Organization: Posted via Supernews, http://www.supernews.com
> To: methods at hgmp.mrc.ac.uk
> Can anyone recommend an assay for total phosholipids in a complex
> sample (natural biological material)?
> We'd prefer easy-to-use (such as a dye blot) and very sensitive. But
> anything will help at this point.
> Thanks for helping!
Dr. Wolfgang Schechinger
Lab N233 (c/o Dr. Steve Roffler)
Institute of Biomedical Sciences
Yen-Chio Yuan Rd. Sec.2
wolfsc at REMOVETHESEANTISPAMCAPITALSibms.sinica.edu.tw
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