Help: RNA Extraction from Paraffin Embedded Tissue
ymwang at mcw.edu
Wed Mar 21 11:59:47 EST 2001
I have a similar question, i.e. how to extract RNA from paraffin embedded tissue
and was wondering if you have gotten any suggestions.
"Anthony C. Hilton" wrote:
> We are currently trying to extract RNA (specifically mRNA) from archived
> paraffin embedded (PE) renal tissue for subsequent use in a Northern blot
> assay. I'm aware of the difficulty in doing this, especially as much of the
> stability of the RNA in the sample is dependent upon the steps taken to
> prevent RNase activity prior to paraffin embedding. We have tried a number
> of extraction kits which, while they accept the shortfall of their products
> in doing so, claim to be able to extract sufficient mRNA from PE tissue for
> a RT-PCR step.
> Has anyone any experience of getting mRNA out of PE tissues and if so what
> method would you recommend. Are there any steps in the RNA extraction
> process which can be successfully optimised to enhances chances of success?
> Any comments welcome as we are running out of ideas and this is putting our
> research 'on hold' at the moment.
> Anthony Hilton
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