Help: RNA Extraction from Paraffin Embedded Tissue

Michael Witty mw132 at mole.bio.cam.ac.uk
Wed Mar 21 12:51:03 EST 2001


I also have a similar question: how to use paraffin embedded tissues (from
plants) for probing with primary and secondary antibodies
(immunohistochemistry).  What is everyones experience of that?  Mike.

> I have a similar question, i.e. how to extract RNA from paraffin embedded tissue
> and was wondering if you have gotten any suggestions.
> Thanks,
> Youmin Wang
> 
> 
> "Anthony C. Hilton" wrote:
> 
> > Hi,
> >
> > We are currently trying to extract RNA (specifically mRNA) from archived
> > paraffin embedded (PE) renal tissue for subsequent use in a Northern blot
> > assay.  I'm aware of the difficulty in doing this, especially as much of the
> > stability of the RNA in the sample is dependent upon the steps taken to
> > prevent RNase activity prior to paraffin embedding.  We have tried a number
> > of extraction kits which, while they accept the shortfall of their products
> > in doing so, claim to be able to extract sufficient mRNA from PE tissue for
> > a RT-PCR step.
> >
> > Has anyone any experience of getting mRNA out of PE tissues and if so what
> > method would you recommend.  Are there any steps in the RNA extraction
> > process which can be successfully optimised to enhances chances of success?
> >
> > Any comments welcome as we are running out of ideas and this is putting our
> > research 'on hold' at the moment.
> >
> > Thanks
> >
> > Anthony Hilton
> 
> ---
> 
> 




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