A few points to make: -make sure that when you use Molecular Probe dyes, you have the right wave length UV bulbs in your transilluminator and the right filter in your camera, otherwise they're no better than eth-br -Sybr Gold dye is even more sensitive than the Sybr green dyes <http://www.biowww.net/forum/read.php?f=1&i=1377&t=1344>