why does EtBr stain also single strand RNA?

Michael L. Sullivan mlsulliv at facstaff.wisc.edu
Thu Mar 22 17:00:30 EST 2001


Without looking it up, here's what I'd expect.  The bases are hydrophobic
enough so that even in single stranded DNA and RNA they are going to want
to stack to minimize their interarctions with water.  Because the bases are
stacked, dyes like EtBr can still intercalate.

Mike


>How can EtBr stain also single strand? Isn't it an intercalant agent? How
>can it stain also DNA and RNA single strand? Thx very much 2 every1.
>Stefano
>gifalone at tin.it
>
>http://web.tiscalinet.it/gifalone
>ICQ 110419911

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