rapid ligation &electroporation

Thomas Nilsson thomas.nilsson at kau.se
Fri Mar 23 11:03:46 EST 2001


Hello,

I am about to do a ligation using the Roche (previously Boehringer) rapid
ligation kit , followed by electroporation of the ligation product. Roches
manual states that the ligation reaction has to be purified with the "High
Pure PCR Product purifiaction kit". Why is this? A component in the
ligation kit interfering with the electroporation? Anybody with experience
of electroporating rapid ligations?

Thomas Nilsson




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