rapid ligation &electroporation

Ashok Aiyar aiyar at ebv.mimnet.northwestern.edu
Fri Mar 23 12:04:26 EST 2001


On Fri, 23 Mar 2001 17:03:46 +0100,
    Thomas Nilsson (thomas.nilsson at kau.se) wrote:
>Hello,
>
>I am about to do a ligation using the Roche (previously Boehringer) rapid
>ligation kit , followed by electroporation of the ligation product. Roches
>manual states that the ligation reaction has to be purified with the "High
>Pure PCR Product purifiaction kit". Why is this? A component in the
>ligation kit interfering with the electroporation?

My guess -- salt.  We routinely "drop-dialyze" our ligations prior to
electroporations.  This works quite well.

Ashok




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