rapid ligation &electroporation

Steven L Goldberg steven.goldberg at bms.com
Fri Mar 23 14:49:22 EST 2001


You can also clean up the reaction by adding 15 ul water and 250 ul 1-butanol
per 10 ul ligation reaction, then centrifuge 10 min, remove liquid, and dry
before dissolving the DNA (usually quite visible).

Thomas Nilsson wrote:

> Hello,
>
> I am about to do a ligation using the Roche (previously Boehringer) rapid
> ligation kit , followed by electroporation of the ligation product. Roches
> manual states that the ligation reaction has to be purified with the "High
> Pure PCR Product purifiaction kit". Why is this? A component in the
> ligation kit interfering with the electroporation? Anybody with experience
> of electroporating rapid ligations?
>
> Thomas Nilsson
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