Lambda phage preparation

Dima Klenchin klenchin at REMOVE_TO_REPLY.facstaff.wisc.edu
Fri Mar 23 15:18:44 EST 2001


dpatil at bioch.ox.ac.uk (Devanagoud Patil) wrote:
:Dear Netters,
:
:
:Is there a filteration based method for large scale preparation of lambda
:phage (instead of CsCl2 gradient centrifugation)?

PEG precipitation will most likely give better purity than any 
filtration-based protocol. It is straightforward scale up of
a traditional lambda miniprep. See, for example
http://www.qiagen.com/literature/handbooks/phage/lambda/1011142_lambda.pdf

        - Dima





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