western blotting

Nick Theodorakis nicholas_theodorakis at urmc.rochester.edu
Fri Mar 23 18:15:24 EST 2001


In <20010323201302.9773.qmail at web4005.mail.yahoo.com>, songsong cao wrote:
>
>I am doing western blotting now. The primary antibody
>I got is serum and I have no idea how many times I
>should dilute it before use. Could you give me some
>suggestions? Thank you.
>


Every antisera is different. I've seen everything from 1:100 to 1:5000.

Try titering it yourself on western "strips" of the same sample.

Nick

-- 
Nick Theodorakis
nicholas_theodorakis at urmc.rochester.edu




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