rapid ligation &electroporation

Dr. Duncan Clark news at hgmp.mrc.ac.uk
Fri Mar 23 12:32:47 EST 2001


In article <thomas.nilsson-2303011703460001 at adm116.kemi.kau.se>, the
eminent Thomas Nilsson at Karlstads Universitet wrote
>Why is this? A component in the
>ligation kit interfering with the electroporation?

Ligase itself is very inhibitory plus the salt in the reaction will
cause the cuvette to arc.

Duncan
-- 
The problem with being on the cutting edge is that you occasionally get 
sliced from time to time....

Duncan Clark
GeneSys Ltd.
Tel: +44(0)1252376288
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http://www.dnamp.com
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