rapid ligation &electroporation

Dr. Duncan Clark news at hgmp.mrc.ac.uk
Fri Mar 23 12:32:47 EST 2001

In article <thomas.nilsson-2303011703460001 at adm116.kemi.kau.se>, the
eminent Thomas Nilsson at Karlstads Universitet wrote
>Why is this? A component in the
>ligation kit interfering with the electroporation?

Ligase itself is very inhibitory plus the salt in the reaction will
cause the cuvette to arc.

The problem with being on the cutting edge is that you occasionally get 
sliced from time to time....

Duncan Clark
GeneSys Ltd.
Tel: +44(0)1252376288
FAX: +44(0)8701640382

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