What antibody stains peroxisome?

lerme at lyon-sud.univ-lyon1.fr lerme at lyon-sud.univ-lyon1.fr
Mon Mar 26 10:20:49 EST 2001


Hi,
In peroxisomes there are structures named uricase or urate oxidase.
we have a rabbit antiserum against urate oxidase, we only used for electron microscopic analyses in mouse hepatocytes. as you want .

Fabienne SIMIAN-LERME
Inserm U. 189
Faculte de medecine lyon-sud
bp 12
69921 OULLINS Cedex, France
tel 33.4.78.86.31.58
fax 33.4.78.50.71.52
lerme at lyon-sud.univ-lyon1.fr

Zhonglin Chai a écrit :

> Dear netters,
>
> I am looking for an antibody to be used as a marker for peroxisome in human cells (HeLa, for example). It will be used in an immunofluorescent (IF) double staining, hence must be applicable for IF and reactive or cross-reactive to peroxisome proteins of human origin. Abs to catalase should be ideal as catalase is in a pretty high level in peroxisomes. Any suggestions and comments on commercial antibodies for this purpose will be highly appreciated.
>
> Zhonglin Chai
>
> Zhonglin Chai, PhD
>
> Hypertension Lab
> Baker Medical Research Institute
> Commercial Rd, Prahran, Victoria
> Australia 3181
> Telephone: +61 3, 9522 4357
> mobile : 0413 58 1940, or international +61, 413 58 1940
> Fax: +61 3, 9521 1362
> email: zhonglin.chai at baker.edu.au
>
> ---




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