Q: EtBr absorbance

TFitzwater at Gilead.com TFitzwater at Gilead.com
Mon Mar 26 13:21:08 EST 2001


>Krzysztof Wasowicz (wasowicz at moskit.uwm.edu.pl)
>Wed 14 Mar 2001 - 13:57:43 GMT>Hi,
>I have an EtBr solution of unknown concentration (between 10 and 20
>mg/ml). Does anyone know what is the specific absorbance of EtBr
>solution and at what wavelength it should be measured? I cannot make a
>reference solution. Any help will be appreciated. Please, e-mail me.
>Krzysztof Wasowicz, DVM, Ph. D.
>Department of Animal Anatomy
>Faculty of Veterinary Medicine
>University of Warmia and Mazury
>ul. Oczapowskiego 13 (Bldg. 105J)
>10-957 Olsztyn-Kortowo
>Poland
>Phone: +48-89-5233688, 5233733
>Fax: +48-89-5234986
>Email: wasowicz at uwm.edu.pl

In late 1994, Amresco published an article in their newsletter that used
A285 to determine the ug/ml concentration of ethidium bromide.  I saved the
graph, but not the article.  You might try their website:
http://www.amresco-inc.com
or their Tech service e-mail:  amrtech at worldnet.att.net

Tim Fitzwater
Associate Scientist
Gilead Sciences

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