rapid ligation &electroporation

Dima Klenchin klenchin at REMOVE_TO_REPLY.facstaff.wisc.edu
Mon Mar 26 15:30:09 EST 2001


hroychow at nmsu.edu ("Dr. Hiranya S. Roychowdhury") wrote:
:Electroporation is possible because DNA molecules carry the charge across
:the electrodes, as projectiles, and are essentially inserted into the cells
:by the electromotive force.  

Nitpicking addition :-)

Electroporation is possible because of what you described _plus_
electropore formation in electric field. Without pores and before they
are formed, field goes around the cell and DNA goes electrophoretically
along it. Once the pores form by a mechanism analogous to dielectric
breakdown of capacitor, electric field goes primarily through the cell 
(conductivity inside is higher) and gets "concentrated" near the pores.
Thus, electrophoresis drives DNA right to the pore and iside the cell.
"Resealable" electropores are quite small, but bilayer is elastic and
electromotive force is strong enough to push huge but flexible 
DNA molecules through.

        - Dima




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