Help! how to get rid of plasmid?

haeock lee at vision.wustl.edu
Tue Mar 27 16:06:04 EST 2001


I've been doind ET cloning in a BAC host strain. To express RecE/T, we used
pBADabg (tetracyclin version) from Francis Stewart. We got the recombination
we wanted. Now, we need to recover the recombined BAC DNA.
The problem is that there's too much pBADabg and I can't get rid of it.
I've restreaked the E. coli without tetracyclin a few times. pBADabg
persists. Does anyone know how to cure this plasmid?


<http://www.biowww.net/forum/read.php?f=1&i=1438&t=1438>




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