rapid ligation &electroporation

Dr. Hiranya S. Roychowdhury hroychow at nmsu.edu
Tue Mar 27 22:10:09 EST 2001


Thanx, Dima, for the additional information. 


At 08:30 PM 3/26/01 GMT, Dima Klenchin wrote:
>hroychow at nmsu.edu ("Dr. Hiranya S. Roychowdhury") wrote:
>:Electroporation is possible because DNA molecules carry the charge across
>:the electrodes, as projectiles, and are essentially inserted into the cells
>:by the electromotive force.  
>
>Nitpicking addition :-)
>
>Electroporation is possible because of what you described _plus_
>electropore formation in electric field. Without pores and before they
>are formed, field goes around the cell and DNA goes electrophoretically
>along it. Once the pores form by a mechanism analogous to dielectric
>breakdown of capacitor, electric field goes primarily through the cell 
>(conductivity inside is higher) and gets "concentrated" near the pores.
>Thus, electrophoresis drives DNA right to the pore and iside the cell.
>"Resealable" electropores are quite small, but bilayer is elastic and
>electromotive force is strong enough to push huge but flexible 
>DNA molecules through.
>
>        - Dima
>
>
Dr. Hiranya Sankar Roychowdhury
College Asst. Prof.
Molecular Biology,
Dept. of Chemistry & Biochemistry	
Box 30001 - 3MLS
New Mexico State University
Las Cruces, NM 88003

Lab: (505) 646 4722
Office: (505) 646 8256
hroychow at nmsu.edu

---




More information about the Methods mailing list