concentrating G3P

Michael Witty mw132 at mole.bio.cam.ac.uk
Wed Mar 28 10:02:16 EST 2001


. . . could freeze drying be a possibility?  Mike.

On Wed, 28 Mar 2001, Eric DUFOUR wrote:

> Hello,
> 
>     I'm trying to make a concentrated solution of DL-Glyceraldheide 3
> phosphate. The protocol I had from sigma to obtain G3P from its
> diethylaceatal form leads to 100 micromoles  of d-G3P in aproximately 8
> ml. I need a 5 times more concentrated solution. Is there any
> alternative protocol or way to concentrate G3P without decomposition?
> 
> Thanks,
> Eric
> 
> PS: the protocol use a resine to witch the diacetal suspended in water
> form attach and a boiling water bath to detach G3P, then sucessive
> washing, centrifugation steps to recover G3P in the supernatant.
> 
> 
> 




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