Invitro mutagenesis

[andinesh]

Hi, 
I have a problem. I have cloned a 1.4kb gene into BamHI site of pSBETa vector, 
I have been trying to do invitro mutagenesis using quickchange mutagenesis kit 
from stratagene, somehow its not working with my plasmid, but it works with 
the control plasmid provided along with the kit. I have tried various 
concentrations of both template as well as primers. 
Could anyone suggest some solutions to this problem, I would be very greatful 
if anyone suggest me some solutions which could solve my problem.

Thanking you

Dinesh
Dept. of Botany
The University of HongKong 
Hongkong
andinesh at hkucc.hku.hk

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