PCR and unexpected products

Errol E.S.Kwan at Massey.ac.nz
Thu Mar 29 19:26:53 EST 2001


There's a PCR manual by Alkami (www.Alkami.com) that says that
decreasing the template concentration increases the primer/template
ratio, which leads to greater efficiency but decreases the specificity
leading to the generation of unspecific amplicons.  This is probably
what you're seeing.  

The Alkami website has a PDF format of this manual and I have found it
very helpful.

On Tue, 27 Mar 2001 21:04:20 +0200, "Stefi~" <xxxgifalone at tin.it>
wrote:

>How could I explain the fact that in a RT-PCR, decreasing RNA template
>quantity in microtube I obtain decreased levels of specific band and
>*constant* levels of aspecific band?
>Thx very much.
>Stefano
>gifalone at tin.it
>
>http://web.tiscalinet.it/gifalone
>ICQ 110419911
>
>




More information about the Methods mailing list