RT-PCR with Tth DNA Pol

Jesús Torres torrespi at ugr.es
Wed Mar 28 09:01:33 EST 2001


Hi all:

    I'm having problems making RT-PCR with Tth Plus DNA Polymerase
(GeneCraft), specially in RT reaction. I use specific 3' primer for
retrotranscription of mRNA. I don't know where is my problem (RNA
concentration, primer concentration, Cl2Mn concentration, annealing
temperature,...). I have made RT-PCR with other polymerases and I never
had any problem. Please, give me some ideas, thank you very much.

Jesus Torres
Dpt. of Biochemistry and Molecular Biology
Faculty of Medicine
University of Granada
Granada, Spain






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