PCR and unexpected products
xxxgifalone at tin.it
Fri Mar 30 07:11:26 EST 2001
Errol <E.S.Kwan at Massey.ac.nz> wrote in message
3ac3d244.1543913410 at news.massey.ac.nz...
> There's a PCR manual by Alkami (www.Alkami.com) that says that
> decreasing the template concentration increases the primer/template
> ratio, which leads to greater efficiency but decreases the specificity
> leading to the generation of unspecific amplicons. This is probably
> what you're seeing.
> The Alkami website has a PDF format of this manual and I have found it
> very helpful.
thanx very much. I'll download this manual.
gifalone at tin.it
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