Ubiquinone for Mitochondrial Complex I Assays?
Paul S. Brookes.
brookes at uab.edu
Fri Mar 30 16:29:13 EST 2001
We're troubleshootiing a spectrophotometric mitochondrial complex I (NADH
ubiquinone oxidoreductase) assay, and suspect that one problem may be the
ubiquinone we're using. It's Sigma Decylubiquinone (cat# D7911) which is
listed as 2,3-dimethoxy-5-methyl-6-decyl-1-4-benzoquinone). I got this a
few months back because they didn't have any Co-enzyme Q2 (cat#
C8081). The two compounds are essentially the same, except the Q2 has
geranyl instead of decyl units. I cannot see how this would affect the
performance of the "business" end of the Q molecule.
So, has anyone squared these two compunds off in a complex I assay? Which
one works best? Is it remotely feasible that our use of the decyl compound
is responsible for the assay not working? We've tried just about
everyhting else. Even the positive control (isolated heart mito's at
1mg/ml in the assay cuvet) will not work.
Dr. Paul S. Brookes. (brookes at uab.edu)
UAB Department of Pathology, G004 Volker Hall
1670 University Blvd., Birmingham AL 35294 USA
Tel (001) 205 934 1915 Fax (001) 205 934 1775
Freudian slip - when you say one thing but mean a mother
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