Ghost bands on plasmid preps

ChenHA hzhen at freeuk.com
Sat Mar 31 20:33:10 EST 2001



jw wrote:
> 
> This ghost band sounds to me like a small amount of supercoiled DNA
> (completely uncut plasmid).  

Unlikely as this is ghost band is apparently a property of pBluescript
when used in XL1-blue  This subject had been extensively discussed and
can be found in the article already mentioned:

ftp://ftp.ncifcrf.gov/pub/methods/TIBS/mar94.txt

The answer is that no one knows.  There is really nothing much to add
unless someone is prepared to do more experiments on that like
sequencing the plasmid, or someone has some new insights into this
particular issue.




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