Ghost bands on plasmid preps
jw
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Sat Mar 31 23:17:19 EST 2001
ChenHA <hzhen at freeuk.com> wrote:
>
> Unlikely as this is ghost band is apparently a property of pBluescript
> when used in XL1-blue This subject had been extensively discussed and
> can be found in the article already mentioned:
>
> ftp://ftp.ncifcrf.gov/pub/methods/TIBS/mar94.txt
>
> The answer is that no one knows. There is really nothing much to add
> unless someone is prepared to do more experiments on that like
> sequencing the plasmid, or someone has some new insights into this
> particular issue.
Thanks for the reference. I found this information very interesting and
helpful.
JW
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