PCR problem

Fabien Jovelin f.jovelin at eurosequence.com
Wed May 2 10:34:34 EST 2001

I have encountered a strange problem doing large amount of PCR :  I have
done a premix with all the composants and the PCR did not work...for the
same DNA, and primers I have done the PCR without premix and it worked
well...it seems there is no problem of appariement and I have done Hot start
any explanation ???


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